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Image Search Results
Journal: Journal of Virology
Article Title: Cytoplasmic Tail Truncation of SARS-CoV-2 Spike Protein Enhances Titer of Pseudotyped Vectors but Masks the Effect of the D614G Mutation
doi: 10.1128/JVI.00966-21
Figure Lengend Snippet: Sensitivity of different Spike proteins to convalescent serum neutralization. Indicated VSV-Luc pseudoviruses were incubated with serially diluted sera (1:10- to 1:7,290-fold) from control or convalescent COVID-19 patients (CCS) for 30 min before addition to HeLa-ACE2 cells. Luciferase activity was determined 24 h later. All values were normalized to the luciferase signal from cells transduced with the same pseudovirus without serum. Means and standard deviations from 3 technical replicates of single vector stocks are shown.
Article Snippet: The S1 subunit of Spike was detected using SARS-CoV-2 (COVID-19) Spike S1 antibody at 1:1,000 (9083;
Techniques: Neutralization, Incubation, Luciferase, Activity Assay, Transduction, Plasmid Preparation